Effect of silver nanoparticles on sterilization of different explant sources of Gerbera jamesonii cultured in vitro

In this study, silver nanoparticles effects on the sterilization of different sources explants (young leaves, young flower stalks and buds) Gerbera as well in vitro morphogenesis their growth were investigated. The sterilized cut transversally (1 mm) with stalk, square (0.5 × 0.5 cm) for leaf sample, longitudinally flowers (removed petals) cultured MS medium; then, (contamination-free or no browning/necrosis) transferred into medium supplemented 0.02 mg/L TDZ plus 0.8 adenine, 10% coconut water, 30 g/L sucrose 8 agar 15 days. results showed that AgNPs at appropriate concentration duration treatment was effective explant bud (0.02% 20 min), min) leaves (0.05% after days culture. addition, 3 types including callus induction, somatic embryogenesis direct shoot regeneration derived from by improved compared to HgCl2. research optimal multiplication, rooting evaluation acclimatization greenhouse also studied. Results 2 NAA combined BA KIN is suitable multiplication; meanwhile, ability quality plantlets improving survival rate vitro.